INTENDED USE

The Relaxin is a specific hormone generated mainly by the placenta. It is usually used as an indicator of pregnancy status in female dogs. In pregnant dogs, relaxin can be detected from 15 days after the nidation of fertilized egg in the uterus wall. The Pregnancy Relaxin RLN Test is a rapid test to detect relaxin in bitches that may be pregnant. The suggested detection period is 22-30 days after mating. Too early detection may lead to a negative result when relaxin in serum is too low. It is suggested to repeat the test after 1 week if a negative result was observed.

PRINCIPLE

The Canine Pregnancy Relaxin (RLN) Test is based on sandwich lateral flow immunochromatographic assay. The test device has a testing window. The testing window has an invisible T (test) zone and C (control) zone. When sample is applied into the sample hole on the device, the liquid will laterally flow on the surface of the test strip. If the concentration of laxin in the sample exceeds the detection limit, a visible T-line will appear. After applying the sample, the c-line should always appear, which indicates the valid result.

KIT COMPONENT

-Test device.

– Dropper

– Product Manual

STORAGE & SHELF LIFE

Store at 2-30°C, out of sun light and moisture.

Shelf Life: 24 months

TEST PROCEDURE

1.Collect cat’s fresh blood and centrifuge it to get serum or plasma. Ensure the samples can’t be turbid or precipitated. Do not leave the specimens at room temperature for long time.. 

2. Take out the test device from the foil pouch and place it horizontally.

3. Vertically drip 3 drops (100ul) sample into the sample hole with dropper.

4. Interpret the result in 5-10 minutes. Result after 15 minutes is considered as invalid.

INTERPRETATION OF RESULTS

Positive: Two lines appear, no matter T line is clear or vague.

Negative: Only C line appears.

Invalid: No line appears or only T line appears.

PRECAUTIONS

1.Recover all the device and specimen to room temperature (15-30°C）before testing. 

2.Do not use tap water, pure water, distilled water, and physiological saline as negative controls.

3. It is only for veterinarian’s quick diagnosis reference and only provides qualitative test results. The diagnosis should be made based on clinical symptoms or other routine tests.

4. The earliest pregnancy days for dogs detected by this product is 15 days.

5. Do not use the expired test or the test with damaged foil pouch.

6. Do not reuse the test kit and do not mix components from different lot numbers.

LIMITATION

Rapid Canine Pregnancy Relaxin (RLN) Test Kit is for in vitro veterinary diagnosis use only. All results should be considered with other clinical information available from veterinarian. For an accurate result, it is suggested to apply other method such as PCR for final determination in practice