INTENDED USE

The Canine Corona Virus Antigen Rapid Test is a lateral flow immunochromatographic assay for the qualitative detection of canine Corona virus antigen (CCV Ag) in dog’s feces specimen.

PRINCIPLE

The Canine Corona Virus Antigen Rapid Test is based on sandwich lateral flow immunochromatographic assay. The test device has a testing window for the observation of assay running and result reading. The testing window has an invisible T (test) zone and a C (control) zone before running the assay. When the treated sample was applied into the sample hole on the device, the liquid will laterally flow through the surface of the test strip and react with the pre-coated monoclonal antibodies. If there is CCV antigen in the specimen, a visible T line will appear. The C line should always appear after a sample is applied, which indicates a valid result. By this means, the device can accurately indicate the presence of Corona virus antigen in the specimen.

KIT COMPONENT

-Test device, with disposable droppers

-Assay buffer  

-Cotton swabs

-Products Manual

STORAGE & SHELF LIFE

Store at 2-30°C, out of sun light and moisture.

Shelf Life: 24 months

TEST PROCEDURE

1. Collect dog’s fresh feces with the cotton swab. Make the swab wet sufficiently. If for screening CCV in dogs without diarrhea, please collect specimen from rectal not feces.

2. Insert the wet swab into the provided assay buffer tubes.  Agitate it at least 10 times to get efficient sample extraction. If the fecal particles are large, let it stand for 1-2 minutes to precipitate.

3. Take out the test device from the foil pouch and place it horizontally.

4. Gradually drip 5 drops of sample extraction into the sample hole with disposable droppers.

5. Interpret the result in 10-15 minutes. Result after 15 minutes is considered as invalid.

INTERPRETATION OF RESULTS

Positive: Two lines appear, no matter T line is clear or vague.

Negative: Only C line appears.

Invalid: No line appears or only T line appears.

PRECAUTIONS

1. The test must be used within 1 hour after opening the foil pouch.

2. Adding too much feces samples will cause false positive and overdosing assay buffer can also lead to incorrect results.

3. Extract samples should be tested immediately.

4. Do not use the expired test or the test with damaged foil pouch.

5. Do not reuse the test kit and do not mix components from different lot numbers.

LIMITATION

SHENUNGTEX Canine Corona Virus Antigen Rapid Test is for in vitro veterinary diagnosis use only. All results should be considered with other clinical information available from veterinarian. For an accurate result, It is suggested to apply other method such as PCR for final determination in practice.